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FLIP Polyclonal Antibody

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF(ICC)
  • IF

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-8855R-100uL
Product Name FLIP Polyclonal Antibody
Applications
WB IHC-P IHC-F IF(ICC) IF
Reactivity Human, Mouse, Rat
Predicted Reactivity Pig, Horse, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CFLIP
Immunogen Range 1-100/480
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 8837
Swiss Prot O15519
Subcellular location Cytoplasm
Synonyms c FLIP; c FLIPL; c FLIPR; c FLIPS; c-FLIP; c-FLIPL; c-FLIPR; c-FLIPS; CASH; CASP8 and FADD like apoptosis regulator; CASP8 and FADD-like apoptosis regulator subunit p12; CASP8AP1; Caspase homolog; Caspase like apoptosis regulatory protein; Caspase related inducer of apoptosis; Caspase-eight-related protein; Caspase-like apoptosis regulatory protein; Casper; Cellular FLICE-like inhibitory protein; CFLA; Cflar; CFLAR_HUMAN; CLARP; FADD like anti apoptotic molecule; FADD-like antiapoptotic molecule 1; FLAME; FLAME-1; FLAME1; FLIP; I FLICE; I-FLICE; Inhibitor of FLICE; MACH-related inducer of toxicity; MRIT; OTTHUMP00000163715; OTTHUMP00000206475; OTTHUMP00000206476; OTTHUMP00000206478; OTTHUMP00000206479; OTTHUMP00000206480; OTTHUMP00000206482; OTTHUMP00000207360; Usurpin; Usurpin beta.
Background The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011]
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500
IF(ICC) 1:50-200
IF

Frequently asked questions

Which applications is FLIP Polyclonal Antibody (bs-8855R) validated for, and at what dilution?

bs-8855R is validated for WB (1:300-5000); IHC-P (1:200-400); IHC-F (1:100-500); IF(ICC) (1:50-200); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does FLIP Polyclonal Antibody (bs-8855R) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Pig, Horse, Rabbit.

How should bs-8855R be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise FLIP Polyclonal Antibody (bs-8855R)?

KLH conjugated synthetic peptide derived from human CFLIP Immunogen range: 1-100/480.

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