Arginase 1 Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated

HepG2 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Arginase 1 Polyclonal Antibody(bs-8585R-AF488)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).