EP4 Polyclonal Antibody

Raji cell lysates probed with EP4 Polyclonal Antibody, Unconjugated (bs-8538R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Mouse Lung tissue lysates; Lane 2: Rat Cerebrum tissue lysates; Lane 3: Rat Esophagus tissue lysates; Lane 4: Rat Liver tissue lysates probed with EP4 Polyclonal Antibody, Unconjugated (bs-8538R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Lung tissue lysates; Lane 2: Rat Cerebrum tissue lysates; Lane 3: Rat Esophagus tissue lysates; Lane 4: Rat Liver tissue lysates probed with EP4 Polyclonal Antibody, Unconjugated (bs-8538R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F

Reactivity

Mouse

Overview
Catalog #	bs-8538R
Product Name	EP4 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F
Reactivity	Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human EP4R/Prostaglandin E Receptor EP4
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5734
Swiss Prot	P35408
Subcellular location	Cell membrane
Synonyms	EP4; EP4R; Prostaglandin E2 receptor EP4 subtype; PGE receptor EP4 subtype; PGE2 receptor EP4 subtype; Prostanoid EP4 receptor; PTGER4; PTGER2
Background	Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500