MHC Class 2 Polyclonal Antibody

Mouse lung lysates probed with Anti MHC Class II Polyclonal Antibody, Unconjugated (bs-8481R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 27kD. Observed band size: 30kD

$Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-MHC Class II Polyclonal Antibody, Unconjugated (bs-8481R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n$

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-MHC Class II Polyclonal Antibody, Unconjugated (bs-8481R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human
Mouse

Predicted Reactivity

Overview
Catalog #	bs-8481R
Product Name	MHC Class 2 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human, Mouse
Predicted Reactivity	Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human HLA class II histocompatibility antigen DM beta chain
Immunogen Range	111-200/263
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3109
Swiss Prot	P28068
Subcellular location	Cytoplasm, Cell membrane
Synonyms	RING7; D6S221E; HLA class II histocompatibility antigen, DM beta chain; MHC class II antigen DMB; Really interesting new gene 7 protein; HLA-DMB; DMB
Background	Plays a critical role in catalyzing the release of class II-associated invariant chain peptide (CLIP) from newly synthesized MHC class II molecules and freeing the peptide binding site for acquisition of antigenic peptides. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500

Applications

Reactivity

Predicted Reactivity

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