P2RX4 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Anti-P2RX4 Polyclonal Antibody, Unconjugated (bs-7690R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Formalin-fixed and paraffin-embedded mouse colon labeled with Anti-P2RX4 Polyclonal Antibody, Unconjugated(bs-7690R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37\u00b0C.$

Formalin-fixed and paraffin-embedded mouse colon labeled with Anti-P2RX4 Polyclonal Antibody, Unconjugated(bs-7690R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37\u00b0C.

$Antigen: bs-7690P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450$

Antigen: bs-7690P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450

$25 ug total protein per lane of various lysates (see on figure) probed with P2RX4 polyclonal antibody, unconjugated (bs-7690R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with P2RX4 polyclonal antibody, unconjugated (bs-7690R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Human
Mouse
Rat
Dog

Overview
Catalog #	bs-7690r-100ul
Product Name	P2RX4 Polyclonal Antibody
Applications	WB, IHC-P, IF(IHC-P)
Specificity	This protein is known to be similar in amino acid sequence to P2RX5, 6 and 7, and may react with these proteins based upon sequence homology. P2RX5 and 7 are expressed at high levels in brain and immune system and P2RX6 is expressed predominantly in skeletal muscle.
Reactivity	Human, Mouse, Rat, Dog
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human P2RX4
Immunogen Range	280-330/388
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	5025
Swiss Prot	Q99571
Subcellular location	Extracellular
Synonyms	P2X4; P2X4R; P2X purinoceptor 4; ATP receptor; Purinergic receptor; P2RX4
Background	Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200