Factor B Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-6949R-100uL |
| Product Name | Factor B Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
IF(IHC-P)
|
| Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Complement factor-B Bb fragment |
| Immunogen Range | 301-400/764 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 629 |
| Swiss Prot | P00751 |
| Subcellular location | Secreted |
| Synonyms | BF; FB; BFD; GBG; CFAB; CFBD; PBF2; AHUS4; FBI12; H2-Bf; ARMD14; Complement factor B; C3/C5 convertase; Glycine-rich beta glycoprotein; Properdin factor B; CFB |
| Background | Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is Factor B Polyclonal Antibody (bs-6949R) validated for, and at what dilution?
bs-6949R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Factor B Polyclonal Antibody (bs-6949R) react with?
Tested reactivity: Mouse, Rat.
How should bs-6949R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Factor B Polyclonal Antibody (bs-6949R)?
KLH conjugated synthetic peptide derived from human Complement factor-B Bb fragment Immunogen range: 301-400/764.