CH25H Polyclonal Antibody

$Lane 1: Human U-87 MG cell Lysates. Probed with CH25H polyclonal Antibody, unconjugated (bs-6480R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human U-87 MG cell Lysates. Probed with CH25H polyclonal Antibody, unconjugated (bs-6480R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$\tParaformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CH25H Polyclonal Antibody, Unconjugated (bs-6480R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

\tParaformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CH25H Polyclonal Antibody, Unconjugated (bs-6480R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Human
Rat

Overview
Catalog #	bs-6480r-TR
Product Name	CH25H Polyclonal Antibody
Applications	WB, IHC-P, IHC-F, IF
Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CH25H
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	9023
Swiss Prot	O95992
Subcellular location	Cytoplasm, Cell membrane
Synonyms	C25H; Cholesterol 25 hydroxylase; Cholesterol 25-hydroxylase; Cholesterol 25 monooxygenase; EC 1.14.99.38; EC=1.14.99.38; h25OH; CH25H_HUMAN.
Application Dilution
WB	=1:500-2000
IHC-P	IHC-P=1:400-800
IHC-F	IHC-F=1:400-800
IF	IF=1:100-500