phospho-Girdin (Ser1417) Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6465R-100uL |
| Product Name | phospho-Girdin (Ser1417) Polyclonal Antibody |
| Applications |
WB
ELISA
FCM
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Horse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Girdin around the phosphorylation site of Ser1417 |
| Modification Site | Ser1417 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 55704 |
| Swiss Prot | Q3V6T2 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | APE; GIV; GRDN; HkRP1; GIRDIN; KIAA1212; Akt phosphorylation enhancer; Coiled-coil domain-containing protein 88A; G alpha-interacting vesicle-associated protein; Girders of actin filament; Hook-related protein 1; CCDC88A |
| Background | Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Enhances phosphoinositide 3-kinase (PI3K)-dependent phosphorylation and kinase activity of AKT1/PKB, but does not possess kinase activity itself. Phosphorylation of AKT1/PKB thereby induces the phosphorylation of downstream effectors GSK3 and FOXO1/FKHR, and regulates DNA replication and cell proliferation (By similarity). Essential for the integrity of the actin cytoskeleton and for cell migration. Required for formation of actin stress fibers and lamellipodia. May be involved in membrane sorting in the early endosome. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is phospho-Girdin (Ser1417) Polyclonal Antibody (bs-6465R) validated for, and at what dilution?
bs-6465R is validated for WB (1:300-5000); ELISA (1:500-1000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Girdin (Ser1417) Polyclonal Antibody (bs-6465R) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse.
How should bs-6465R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Girdin (Ser1417) Polyclonal Antibody (bs-6465R)?
KLH conjugated synthetic phosphopeptide derived from human Girdin around the phosphorylation site of Ser1417