| Overview |
| bs-6460R |
| Mycobacterium tuberculosis Ag85A/B Antibody |
| ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC) |
| This antibody will detect both Mycobacterium tuberculosis Ag85A and Mycobacterium tuberculosis Ag85B |
| Bacteria |
| Specifications |
| Unconjugated |
| Rabbit |
| KLH conjugated synthetic peptide derived from Mycobacterium tuberculosis Antigen 85 complex B |
| 115-154/325 |
| Polyclonal |
| IgG |
| 1ug/ul |
| Purified by Protein A. |
| 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Target |
| 885785, 886132 |
| P9WQP1, P9WQP3 |
| Secreted |
| M.tuberculosis;Antigen 85 complex B;30 kDa extracellular protein;Mycobacterium;Mycolyl transferase 85B; A85B_MYCTU;Antigen 85-B; 30 kDa extracellular protein; 85B; Ag85B; Extracellular alpha-antigen; Fibronectin-binding protein B. |
| The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM |
| Application Dilution |
| ELISA |
1:500-1000 |
| IHC-P |
1:200-400 |
| IHC-F |
1:100-500 |
| IF(IHC-P) |
1:50-200 |
| IF(IHC-F) |
1:50-200 |
| IF(ICC) |
1:50-200 |
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