TRP12/TRPV4 Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6425r-100uL |
| Product Name | TRP12/TRPV4 Polyclonal Antibody |
| Applications |
FCM
IHC-P
IHC-F
IF
FLOW-CYT
ICC/IF
|
| Reactivity | Human, Bovine |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Chicken |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TRPV4 |
| Immunogen Range | 301-400/871 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 59341 |
| Swiss Prot | Q9HBA0 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | SMAL; VRL2; BCYM3; CMT2C; SPSMA; TRP12; VROAC; HMSN2C; OTRPC4; SSQTL1; Transient receptor potential cation channel subfamily V member 4; TrpV4; Osm-9-like TRP channel 4; Transient receptor potential protein 12; Vanilloid receptor-like channel 2; Vanilloid receptor-like protein 2; VRL-2; Vanilloid receptor-related osmotically-activated channel; VR-OAC |
| Background | Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8. |
| Application Dilution | |
| FCM | 1:20-100 |
| IHC-P | IHC-P=1:100-500 |
| IHC-F | IHC-F=1:100-500 |
| IF | IF=1:100-500 |
| Flow-Cyt | Flow-Cyt=1ug/test |
| ICC/IF | ICC/IF=1:100 |
Frequently asked questions
Which applications is TRP12/TRPV4 Polyclonal Antibody (bs-6425R) validated for, and at what dilution?
bs-6425R is validated for FCM (1:20-100); IHC-P (IHC-P=1:100-500); IHC-F (IHC-F=1:100-500); IF (IF=1:100-500); Flow-Cyt (Flow-Cyt=1ug/test); ICC/IF (ICC/IF=1:100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TRP12/TRPV4 Polyclonal Antibody (bs-6425R) react with?
Tested reactivity: Human, Bovine. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Chicken.
How should bs-6425R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TRP12/TRPV4 Polyclonal Antibody (bs-6425R)?
KLH conjugated synthetic peptide derived from human TRPV4 Immunogen range: 301-400/871.