IL-6 Polyclonal Antibody

Name: IL-6 Polyclonal Antibody
Brand: Bioss
SKU: bs-6309R
Price: 375.0 USD
Availability: InStock

$Mouse lung ) lysates probed with Anti-IL-6 Polyclonal Antibody, Unconjugated (bs-6309R) at 1:300 overnight at 4\u02daC. Followed by a conjugated secondary antibody (bs-0295G-HRP ) at 1:5000 for 90 min at 37\u02daC.$

Mouse lung ) lysates probed with Anti-IL-6 Polyclonal Antibody, Unconjugated (bs-6309R) at 1:300 overnight at 4\u02daC. Followed by a conjugated secondary antibody (bs-0295G-HRP ) at 1:5000 for 90 min at 37\u02daC.

$Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-6 Polyclonal Antibody, Unconjugated (bs-6309R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-6 Polyclonal Antibody, Unconjugated (bs-6309R) at 1:400 overnight at 4\u00b0C, DAB staining.

$HUVEC (H) cells were treated with or without LPS (0.5 \u03bcg\/ml\uff0c24h) and Brefeldin A (300 ng\/ml\uff0c20h) , 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with IL-6 polyclonal antibody, unconjugated (bs-6309R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

HUVEC (H) cells were treated with or without LPS (0.5 \u03bcg\/ml\uff0c24h) and Brefeldin A (300 ng\/ml\uff0c20h) , 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with IL-6 polyclonal antibody, unconjugated (bs-6309R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Mouse
Rat

Overview
Catalog #	bs-6309r
Product Name	IL-6 Polyclonal Antibody
Applications	WB IHC-P IF(IHC-P)
Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from mouse IL-6
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	16193
Synonyms	Interleukin BSF 2; B cell dferentiation factor; B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL dferentiation factor; Cytotoxic T cell dferentiation factor; Hepatocyte stimulating factor; HGF; HPGF; HSF; Hybridoma growth factor; Hybridoma plasmacytoma growth factor; NB2; IL 6; IL6 protein; Interferon beta 2; Interleukin 6 interferon beta 2; Interleukin-6; Interleukin6; IL6_HUMAN.
Background	IL6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. It acts upon a variety of cells including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. In addition, IL6 appears to interact with IL2 in the proliferation of T lymphocytes. IL6 potentiates the proliferative effect of IL3 on multipotential hematopoietic progenitors.
Application Dilution
WB
IHC-P
IF(IHC-P)

Recombinant Proteins (4).png__PID:b4703663-ad26-4eba-90ef-48f549942e61

bs-6309r Copy

Data Sheet