CD16 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-6028R-Cy3-100uL |
| Product Name | CD16 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CD16a |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2214 |
| Swiss Prot | P08637 |
| Subcellular location | Secreted, Cell membrane |
| Synonyms | CD16; FCG3; CD16A; FCGR3; IGFR3; IMD2; FCR-1; FCRIII; FCGRIII; FCRIIIA; Low affinity immunoglobulin gamma Fc region receptor III-A; CD16a antigen; Fc-gamma RIII-alpha; Fc-gamma RIII; Fc-gamma RIIIa; IgG Fc receptor III-2; FCGR3A; FCGR3B; CD16B; |
| Background | Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CD16 Polyclonal Antibody, Cy3 Conjugated (bs-6028R-Cy3) validated for, and at what dilution?
bs-6028R-Cy3 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD16 Polyclonal Antibody, Cy3 Conjugated (bs-6028R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-6028R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD16 Polyclonal Antibody, Cy3 Conjugated (bs-6028R-Cy3)?
KLH conjugated synthetic peptide derived from human CD16a
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-6028R-Cy3)?
Cy3 on bs-6028R-Cy3: excitation / emission 512,550nm/570,615nm.