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Endomucin Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Endomucin Polyclonal Antibody, Unconjugated (bs-5884R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Endomucin Polyclonal Antibody, Unconjugated (bs-5884R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Lane 1: Mouse heart lysates; Lane 2: Mouse lung lysates probed with Endomucin Polyclonal Antibody, Unconjugated (bs-5884R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse heart lysates; Lane 2: Mouse lung lysates probed with Endomucin Polyclonal Antibody, Unconjugated (bs-5884R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Endomucin) polyclonal Antibody, Unconjugated (bs-5884R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Endomucin) polyclonal Antibody, Unconjugated (bs-5884R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Dog
  • Cow
Overview
Catalog # bs-5884R
Product Name Endomucin Polyclonal Antibody
Applications ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Rat, Dog, Cow
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Endomucin
Immunogen Range 181-261/261
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 51705
Swiss Prot Q9ULC0
Subcellular location Secreted, Cell membrane
Synonyms EMCN2; MUC14; Endomucin; Endomucin-2; Gastric cancer antigen Ga34; Mucin-14; MUC-14; EMCN
Background Endothelial sialomucin, also called endomucin or mucin-like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix.
Application Dilution
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

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