CTHRC1 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat skin tissue labeled with Anti CTHRC1 Polyclonal Antibody, Unconjugated (bs-5882R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded smooth muscle of rat colon labeled with Anti CTHRC1 Polyclonal Antibody, Unconjugated (bs-5882R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

L1 rat kidney lysates L2 rat brain lysates probed with Anti CTHRC1 Polyclonal Antibody, Unconjugated (bs-5882R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 20kD. Observed band size: 20kD

$Formalin-fixed and paraffin embedded rat colitis tissue labeled with Anti CTHRC1 Polyclonal Antibody, Unconjugated (bs-5882R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI staining$

Formalin-fixed and paraffin embedded rat colitis tissue labeled with Anti CTHRC1 Polyclonal Antibody, Unconjugated (bs-5882R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI staining

$Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CTHRC1) Polyclonal Antibody, Unconjugated (bs-5882R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CTHRC1) Polyclonal Antibody, Unconjugated (bs-5882R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Cow
Horse
Rabbit

Overview
Catalog #	bs-5882R
Product Name	CTHRC1 Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity	This antibody will recognize all three isoform variants of this protein.
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Cow, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CTHRC1
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	115908
Swiss Prot	Q96CG8
Subcellular location	Secreted, Extracellular matrix
Synonyms	Collagen triple helix repeat-containing protein 1; Protein NMTC1; CTHRC1; UNQ762/PRO1550
Background	CTHRC1 is a protein that may play a role in the cellular response to arterial injury through involvement in vascular remodeling. Mutations at this locus have been associated with Barrett esophagus and esophageal adenocarcinoma. Alternatively spliced transcript variants have been described.
Application Dilution
ELISA	WB1:300-5000
IHC-P	ELISA1:500-1000
IHC-F	IHC-P1:200-400
IF(IHC-P)	IHC-F1:100-500
IF(IHC-F)	IFIHC-P1:50-200
IF(ICC)	IFIHC-F1:50-200