MGAT5 Polyclonal Antibody

HepG2 cell lysates probed with MGAT5 Polyclonal Antibody, Unconjugated (bs-5841R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (MGAT5) Polyclonal Antibody, Unconjugated (bs-5841R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (MGAT5) Polyclonal Antibody, Unconjugated (bs-5841R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

$Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (MGAT5) Polyclonal Antibody, Unconjugated (bs-5841R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (MGAT5) Polyclonal Antibody, Unconjugated (bs-5841R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Chicken
Rabbit

Overview
Catalog #	bs-5841R
Product Name	MGAT5 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human MGAT5
Immunogen Range	201-300/741
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4249
Subcellular location	Cytoplasm, Cell membrane
Synonyms	6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A; 6-N-acetylglucosaminyltransferase; Alpha 1,36 mannosylglycoprotein; Alpha 1,6 mannosylglycoprotein 6 beta N acetylglucosaminyltransferase; Alpha mannoside beta 1,6 N acetylglucosaminyltransferase; Alpha mannoside beta 1,6 N acetylglucosaminyltransferase; Alpha- mannoside beta-1,6-N-acetylglucosaminyltransferase; Alpha-1; alpha-1,36-mannosylglycoprotein; Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase; Alpha-mannoside beta-1; alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase; Beta 1,6 N acetyl glucosaminyltransferase; beta-1,6-N-acetyl-glucosaminyltransferase; GGNT5; GlcNAc T V; GlcNAc-T V; GNT V; GNT VA; GNT-V; GNTV; GNTVA; Mannoside acetylglucosaminyltransferase 5; Mannosyl alpha 1,6 glycoprotein beta 1,6 N acetyl glucosaminyltransferase; mannosyl alpha-1,6--glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase; Mgat5; MGT5A_HUMAN; N acetylglucosaminyl transferase V; N acetylglucosaminyltransferase V mannosyl alpha 1,6 glycoprotein; N-acetylglucosaminyl-transferase V; N-acetylglucosaminyltransferase V.
Background	MGAT5 is a mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, a glycosyltransferase involved in the synthesis of protein-bound and lipid-bound oligosaccharides. Alterations of the oligosaccharides on cell surface glycoproteins cause significant changes in the adhesive or migratory behavior of a cell. Increase in the encoded protein's activity may correlate with the progression of invasive malignancies.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200

Applications

Reactivity

Predicted Reactivity

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