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Lactoferrin Polyclonal Antibody

Human breast milk probed with Lactoferrin Polyclonal Antibody, unconjugated (bs-5810R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\\n
Human breast milk probed with Lactoferrin Polyclonal Antibody, unconjugated (bs-5810R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\\n
Human Lactoferrin protein (100ng) lysates probed with Lactoferrin Polyclonal Antibody, unconjugated (bs-5810R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Human Lactoferrin protein (100ng) lysates probed with Lactoferrin Polyclonal Antibody, unconjugated (bs-5810R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Lactoferrin Polyclonal Antibody(bs-5810R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Lactoferrin Polyclonal Antibody(bs-5810R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Applications

  • WB
  • ELISA
  • FCM
  • IHC-P

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
Overview
Catalog # bs-5810R
Product Name Lactoferrin Polyclonal Antibody
Applications WB, ELISA, FCM, IHC-P
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Sheep, Pig, Horse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Lactoferrin
Immunogen Range 611-710/710
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 4057
Subcellular location Cytoplasm, Nucleus, Secreted
Synonyms CKRX; GIG12; Growth inhibiting protein 12; HLF2; Homo sapiens chemokine receptor X; Kaliocin 1; Lactoferrin; Lactoferroxin A; Lactoferroxin B; Lactoferroxin C; Lactoferroxin-C; Lactotransferrin; LF; LTF; Neutrophil lactoferrin; Talalactoferrin alfa; Talalactoferrin; TRFL_HUMAN.
Background Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400

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