TAGLN2 Polyclonal Antibody

Lane 1: 293T cell lysates; Lane 2: TAGLN2 knockout (KO) 293T cell lysates probed with TAGLN2 Polyclonal Antibody, Unconjugated (bs-55211R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: 293T cell lysates; Lane 2: Hela cell lysates; Lane 3: THP-1 cell lysates; Lane 4: MCF-7 cell lysates; Lane 5: SKOV3 cell lysates; Lane 6: Mouse Lung lysates probed with TAGLN2 Polyclonal Antibody, Unconjugated (bs-55211R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (TAGLN2 ) polyclonal Antibody, Unconjugated (bs-55211R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (TAGLN2 ) polyclonal Antibody, Unconjugated (bs-55211R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
ELISA
IF(ICC)
IF

Reactivity

Human
Mouse

Overview
Catalog #	bs-55211R
Product Name	TAGLN2 Polyclonal Antibody
Applications	WB, ELISA, IF(ICC), IF
Specificity	KO-Validated
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human TAGLN2 (NP_003555.1).
Immunogen Range	1-199/199
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	8407
Swiss Prot	P37802
Subcellular location	Cytoplasm, Secreted, Extracellular matrix
Synonyms	TAGLN2;HA1756
Background	The protein encoded by this gene is similar to the protein transgelin, which is one of the earliest markers of differentiated smooth muscle. The specific function of this protein has not yet been determined, although it is thought to be a tumor suppressor. Multiple transcript variants encoding different isoforms have been found for this gene.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IF(ICC)	1:50-200
IF