SMARCA4 Polyclonal Antibody

Lane 1: 293T cell lysates; Lane 2: SMARCA4 knockout (KO) 293T cell lysates probed with SMARCA4 Polyclonal Antibody, Unconjugated (bs-55203R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: OVCAR3 cell lysates; Lane 2: HeLa cell lysates probed with SMARCA4 Polyclonal Antibody, Unconjugated (bs-55203R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SMARCA4 Polyclonal Antibody, Unconjugated (bs-55203R) at 1:100 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SMARCA4 Polyclonal Antibody, Unconjugated (bs-55203R) at 1:100 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SMARCA4 Polyclonal Antibody, Unconjugated (bs-55203R) at 1:100 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SMARCA4 Polyclonal Antibody, Unconjugated (bs-55203R) at 1:100 overnight at 4\u00b0C, DAB staining.

Applications

WB
ELISA
IHC-P

Reactivity

Human

Overview
Catalog #	bs-55203R
Product Name	SMARCA4 Polyclonal Antibody
Applications	WB, ELISA, IHC-P
Specificity	KO-Validated
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	A synthetic peptide corresponding to a sequence within amino acids 30-130 of human SMARCA4 (NP_003063.2).
Immunogen Range	30-130/1647
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	6597
Swiss Prot	P51532
Subcellular location	Nucleus
Synonyms	BAF190;BAF190A;BRG1;CSS4;MRD16;RTPS2;SNF2;SNF2L4;SNF2LB;SWI2;hSNF2b;SMARCA4
Background	The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400