RHOC Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-55187R-100uL |
| Product Name | RHOC Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-F
IF(ICC)
IF
IHC-P
|
| Specificity | KO-Validated |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human RHOC (NP_001036144.1). |
| Immunogen Range | 1-193/193 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 389 |
| Swiss Prot | P08134 |
| Subcellular location | Cell membrane |
| Synonyms | RHOC;ARH9;ARHC;H9;RHOH9 |
| Background | This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-F | 1:100-500 |
| IF(ICC) | 1:50-200 |
| IF | 1:50-200 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is RHOC Polyclonal Antibody (bs-55187R) validated for, and at what dilution?
bs-55187R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-F (1:100-500); IF(ICC) (1:50-200); IF (1:50-200); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RHOC Polyclonal Antibody (bs-55187R) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.
How should bs-55187R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RHOC Polyclonal Antibody (bs-55187R)?
Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human RHOC (NP_001036144.1). Immunogen range: 1-193/193.