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PDCD4 Polyclonal Antibody

Lane 1: Hela cell lysates; Lane 2: PDCD4 knockout (KO) HeLa cell lysates probed with PDCD4 Polyclonal Antibody, Unconjugated (bs-55166R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: PDCD4 knockout (KO) HeLa cell lysates probed with PDCD4 Polyclonal Antibody, Unconjugated (bs-55166R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: MCF-7 cell lysates; Lane 2: Hela cell lysates; Lane 3: HepG2 cell lysates; Lane 4: Mouse Thymus lysates probed with PDCD4 Polyclonal Antibody, Unconjugated (bs-55166R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: MCF-7 cell lysates; Lane 2: Hela cell lysates; Lane 3: HepG2 cell lysates; Lane 4: Mouse Thymus lysates probed with PDCD4 Polyclonal Antibody, Unconjugated (bs-55166R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • ELISA

Reactivity

  • Human

Predicted Reactivity

  • Mouse
Overview
Catalog # bs-55166R
Product Name PDCD4 Polyclonal Antibody
Applications WB, ELISA
Specificity KO-Validated
Reactivity Human
Predicted Reactivity Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PDCD4 (NP_055271.2).
Immunogen Range 1-260/469
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 27250
Swiss Prot Q53EL6
Subcellular location Cytoplasm, Nucleus
Synonyms PDCD4;H731
Background This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000

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