p21 Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-55160R-100uL |
| Product Name | p21 Polyclonal Antibody |
| Applications |
WB
IHC-F
IF
IHC-P
FLOW-CYT
ICC/IF
|
| Specificity | KO-Validated |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant human p21 |
| Immunogen Range | 1-164/164 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 1026 |
| Swiss Prot | P38936 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | CAP20; CDKN1; CIP1; MDA-6; P21; SDI1; WAF1; p21CIP1; CDKI; mda6; p21WAF; UV96; CDN1A_HUMAN; CDKN1A; CDK-interacting protein 1; Melanoma differentiation-associated protein 6 (MDA-6); PIC1; CDN1A_MOUSE; Melanoma differentiation-associated protein; cyclin dependent kinase inhibitor 1A; cyclin-dependent kinase inhibitor 1A (p21, Cip1) |
| Background | This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene. |
| Application Dilution | |
| WB | 1:500-2000 |
| IHC-F | 1:50-200 |
| IF | 1:50-200 |
| IHC-P | 1:50-200 |
| Flow-Cyt | 1:50-100 |
| ICC/IF | 1:50-200 |
Frequently asked questions
Which applications is p21 Polyclonal Antibody (bs-55160R) validated for, and at what dilution?
bs-55160R is validated for WB (1:500-2000); IHC-F (1:50-200); IF (1:50-200); IHC-P (1:50-200); Flow-Cyt (1:50-100); ICC/IF (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does p21 Polyclonal Antibody (bs-55160R) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should bs-55160R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise p21 Polyclonal Antibody (bs-55160R)?
Recombinant human p21 Immunogen range: 1-164/164.