NDRG1 Polyclonal Antibody

$Lane 1: 293T cell lysates; Lane 2: NDRG1 knockout (KO) 293T cell lysates probed with NDRG1 Polyclonal Antibody, Unconjugated (bs-55141R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: 293T cell lysates; Lane 2: NDRG1 knockout (KO) 293T cell lysates probed with NDRG1 Polyclonal Antibody, Unconjugated (bs-55141R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

WB
ELISA

Reactivity

Human

Predicted Reactivity

Mouse
Rat

Overview
Catalog #	bs-55141R
Product Name	NDRG1 Polyclonal Antibody
Applications	WB ELISA
Specificity	KO-Validated
Reactivity	Human
Predicted Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 175-394 of human NDRG1 (NP_001128714.1).
Immunogen Range	175-394/394
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	10397
Swiss Prot	Q92597
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	NDRG1;CAP43;CMT4D;DRG-1;DRG1;GC4;HMSNL;NDR1;NMSL;PROXY1;RIT42;RTP;TARG1;TDD5
Background	This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000