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N-Cadherin Polyclonal Antibody

Lane 1: Hela whole cell lysates; Lane 2: Hela membrane protein extract probed with N-Cadherin Polyclonal Antibody, Unconjugated (bs-55138R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela whole cell lysates; Lane 2: Hela membrane protein extract probed with N-Cadherin Polyclonal Antibody, Unconjugated (bs-55138R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: N-Cadherin knockout (KO) HeLa cell lysates probed with N-Cadherin Polyclonal Antibody, Unconjugated (bs-55138R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: N-Cadherin knockout (KO) HeLa cell lysates probed with N-Cadherin Polyclonal Antibody, Unconjugated (bs-55138R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: A549 cell lysates; Lane 3: 293T cell lysates; Lane 4: Mouse Liver lysates; Lane 5: Mouse Brain lysates; Lane 6: Mouse Heart lysates; Lane 7: Rat Liver lysates; Lane 8: Rat Heart lysates probed with N-Cadherin Polyclonal Antibody, Unconjugated (bs-55138R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: A549 cell lysates; Lane 3: 293T cell lysates; Lane 4: Mouse Liver lysates; Lane 5: Mouse Brain lysates; Lane 6: Mouse Heart lysates; Lane 7: Rat Liver lysates; Lane 8: Rat Heart lysates probed with N-Cadherin Polyclonal Antibody, Unconjugated (bs-55138R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • ELISA

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
Overview
Catalog # bs-55138R
Product Name N-Cadherin Polyclonal Antibody
Applications WB, ELISA
Specificity KO-Validated
Reactivity Human, Mouse
Predicted Reactivity Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source A synthetic peptide corresponding to a sequence within amino acids 750-850 of human N-Cadherin (NP_001783.2).
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 1000
Swiss Prot P19022
Subcellular location Cell membrane
Synonyms CD325;CDHN;CDw325;NCAD;N Cadherin;CDH2
Background This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000

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