DNMT1 Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-55056R-100uL |
| Product Name | DNMT1 Polyclonal Antibody |
| Applications |
WB
IF(ICC)
IP
IF
|
| Specificity | KO-Validated |
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human DNMT1 (NP_001370.1). |
| Immunogen Range | 1-270/1616 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 1786 |
| Swiss Prot | P26358 |
| Subcellular location | Nucleus |
| Synonyms | ADCADN;AIM;CXXC9;DNMT;HSN1E;MCMT;m.HsaI;DNMT1 |
| Background | This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IP | 1-2ug |
| IF | 1:50-200 |
Frequently asked questions
Which applications is DNMT1 Polyclonal Antibody (bs-55056R) validated for, and at what dilution?
bs-55056R is validated for WB (1:300-5000); IF(ICC) (1:50-200); IP (1-2ug); IF (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DNMT1 Polyclonal Antibody (bs-55056R) react with?
Tested reactivity: Human.
How should bs-55056R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DNMT1 Polyclonal Antibody (bs-55056R)?
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human DNMT1 (NP_001370.1). Immunogen range: 1-270/1616.