Beclin 1 Polyclonal Antibody

Name: Beclin 1 Polyclonal Antibody
Brand: Bioss
SKU: bs-55024R
Price: 375.0 USD
Availability: InStock

$25 ug total protein per lane of various lysates (see on figure) probed with Beclin 1 polyclonal antibody, unconjugated (bs-55024R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Beclin 1 polyclonal antibody, unconjugated (bs-55024R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Beclin 1 Polyclonal Antibody, Unconjugated (bs-55024R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Applications

WB
IHC-F
IF
IHC-P

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Rabbit

Overview
Catalog #	bs-55024R-100uL
Product Name	Beclin 1 Polyclonal Antibody
Applications	WB IHC-F IF IHC-P
Specificity	KO-Validated
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human BECN1
Immunogen Range	181-280/450
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8678
Swiss Prot	Q14457
Subcellular location	Cytoplasm, Cell membrane
Synonyms	ATG6; VPS30; beclin1; BECN1_HUMAN; BECN1; Coiled-coil myosin-like BCL2-interacting protein; Protein GT197; GT197; BECN1_MOUSE; BECN1_RAT; beclin 1; beclin 1 (coiled-coil, moesin-like BCL2 interacting protein); beclin 1, autophagy related; ATG6 autophagy related 6 homolog (S. cerevisiae)
Background	This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.
Application Dilution
WB	1:500-1000
IHC-F	1:400-800
IF	1:100-500
IHC-P	1:400-800

Frequently asked questions

Which applications is Beclin 1 Polyclonal Antibody (bs-55024R) validated for, and at what dilution?

bs-55024R is validated for WB (1:500-1000); IHC-F (1:400-800); IF (1:100-500); IHC-P (1:400-800). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Beclin 1 Polyclonal Antibody (bs-55024R) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Rabbit.

How should bs-55024R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Beclin 1 Polyclonal Antibody (bs-55024R)?

KLH conjugated synthetic peptide derived from human BECN1 Immunogen range: 181-280/450.

Antibodies

Antibodies

Assays & Kits

Proteins & Reagents

In Vivo Reagents

Discovery Tools

Research Tools

Learning

Quick Paths

Beclin 1 Polyclonal Antibody

Applications

Conjugation Options

Reactivity

Predicted Reactivity

BiossPM Rainbow Protein Marker (10~180 kDa)

BiossPM Rainbow Protein Marker (10~245 kDa)

BiossPM Rainbow Protein Marker (10~310 kDa)

Frequently asked questions

Search our catalog of hundreds of thousands of products

Try a recombinant sample

Need a secondary antibody?