IGF1R (Tyr980) Polyclonal Antibody

Formalin-fixed and paraffin embedded human kidney carcinoma labeled with Anti phospho-IGF1R(Tyr980) Polyclonal Antibody,Unconjugated (bs-5447R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human kidney carcinoma labeled with Anti phospho-IGF1R(Tyr980) Polyclonal Antibody,Unconjugated (bs-5447R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IGF1R (Tyr980) Antibody(bs-5447R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IGF1R (Tyr980) Antibody(bs-5447R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IGF1R (Tyr980) Polyclonal Antibody(bs-5447R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IGF1R (Tyr980) Polyclonal Antibody(bs-5447R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse

Predicted Reactivity

Rat

Overview
Catalog #	bs-5447R
Product Name	IGF1R (Tyr980) Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse
Predicted Reactivity	Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human IGF1R around the phosphorylation site of Tyr980
Modification Site	Tyr980
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3480
Swiss Prot	P08069
Subcellular location	Cell membrane
Synonyms	IGFR; CD221; IGFIR; JTK13; Insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; IGF-I receptor; IGF1R
Background	When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200

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