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Ep300 (Ser1834) Polyclonal Antibody

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti-Ep300 (Ser1834) Polyclonal Antibody, Unconjugated (bs-5339R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti-Ep300 (Ser1834) Polyclonal Antibody, Unconjugated (bs-5339R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Hela cell lysates probed with Ep300 Polyclonal Antibody, Unconjugated (bs-5339R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell lysates probed with Ep300 Polyclonal Antibody, Unconjugated (bs-5339R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Zebrafish

Predicted Reactivity

  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
  • Chicken
  • Guinea Pig
Overview
Catalog # bs-5339R
Product Name Ep300 (Ser1834) Polyclonal Antibody
Applications WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Human, Mouse, Zebrafish
Predicted Reactivity Rat, Dog, Cow, Pig, Horse, Chicken, Guinea Pig
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human Ep300 around the phosphorylation site of Ser1834
Modification Site Ser1834
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 2033
Subcellular location Cytoplasm, Nucleus
Synonyms CREBBP/EP300 inhibitory protein 1; Cyclic AMP responsive enhancer binding protein; E1A associated protein p300; E1A binding protein p300; EC 2.3.1.48; EP300; EP300: E1A binding protein p300; RB and P300 binding protein EID 1; Histone acetyltransferase p300; p300 HAT; Retinoblastoma protein associated protein.
Background KAT3B/p300 is a transcriptional adapter protein (300 kDa)which is characterized by three cysteine and histidine rich regions and its C-terminus specifically binds the adenovirus E1A protein. KAT3B and associated proteins are components of TATA-binding protein (TBP)complexes. Protein kinase A mediated CREB phosphorylation results in the binding of CREB to a 265 kDa nuclear protein designated KAT3A/CBP(for CREB-binding protein). KAT3B and KAT3A are homologous to each other.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

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