PADI4 Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-53047R-100uL |
| Product Name | PADI4 Polyclonal Antibody |
| Applications |
WB
ELISA
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Polyclonal antibody raised in rabbit against human PADI4 (Protein-arginine deiminase type-4), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminus.of the protein |
| Clonality | Polyclonal |
| Isotype | Not Determined |
| Concentration | Not Determined |
| Purification | Whole antiserum from rabbit containing 0.05% azide |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 23569 |
| Swiss Prot | Q9UM07 |
| Synonyms | Protein-arginine deiminase type-4, PADI4, PAD4, PADI5, PDI5 |
| Background | Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54Ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54Ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54Ci. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
Frequently asked questions
Which applications is PADI4 Polyclonal Antibody (bs-53047R) validated for, and at what dilution?
bs-53047R is validated for WB (1:300-5000); ELISA (1:500-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PADI4 Polyclonal Antibody (bs-53047R) react with?
Tested reactivity: Human.
How should bs-53047R be stored?
Store at -20C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PADI4 Polyclonal Antibody (bs-53047R)?
Polyclonal antibody raised in rabbit against human PADI4 (Protein-arginine deiminase type-4), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminus.of the protein