CXCL12 Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat pancreas labeled with Anti-SDF-1\/CXCL12 Polyclonal Antibody, Unconjugated (bs-4938R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded rat pancreas labeled with Anti-SDF-1\/CXCL12 Polyclonal Antibody, Unconjugated (bs-4938R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Lane 1: Mouse Liver tissue lysates; Lane 2: Human HT-1080 tissue lysates; Lane 3: Human A549 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human MCF-7 cell lysates; Lane 6: Human HepG2 cell lysates probed with CXCL12 Polyclonal Antibody, Unconjugated (bs-4938R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Liver tissue lysates; Lane 2: Human HT-1080 tissue lysates; Lane 3: Human A549 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human MCF-7 cell lysates; Lane 6: Human HepG2 cell lysates probed with CXCL12 Polyclonal Antibody, Unconjugated (bs-4938R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Pig
Chicken
Rabbit
GuineaPig

Overview
Catalog #	bs-4938R
Product Name	CXCL12 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Pig, Chicken, Rabbit, GuineaPig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human SDF-1/CXCL12
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6387
Swiss Prot	P48061
Subcellular location	Secreted
Synonyms	IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12; Stromal cell-derived factor 1; SDF-1; hSDF-1; C-X-C motif chemokine 12; Intercrine reduced in hepatomas; hIRH; Pre-B cell growth-stimulating factor; CXCL12; SDF1A; SDF1B
Background	Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200