HPV16 E6 Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-49102r-100uL |
| Product Name | HPV16 E6 Polyclonal Antibody |
| Applications |
WB
IHC-P
IHC-F
IF
FLOW-CYT
|
| Reactivity | Human, HPV16 |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant HPV16 E6 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 1489078 |
| Swiss Prot | P03126 |
| Synonyms | E6; HPV16 E6 protein; Human Papilloma Virus; Human papillomavirus type 16 E6; Human papillomavirus type 16; Protein E6; E6 protein; HPV16 E6; HPV16-E6; VE6_HPV16. |
| Background | Plays a major role in the induction and maintenance of cellular transformation. Acts mainly as an oncoprotein by stimulating the destruction of many host cell key regulatory proteins. E6 associates with host E6-AP ubiquitin-protein ligase, and inactivates tumor suppressors TP53 and TP73 by targeting them to the 26S proteasome for degradation. In turn, DNA damage and chromosomal instabilities increase and lead to cell proliferation and cancer development. The complex E6/E6P targets several other substrates to degradation via the proteasome including host NFX1-91, a repressor of human telomerase reverse transcriptase (hTERT). The resulting increased expression of hTERT prevents the shortening of telomere length leading to cell immortalization. Other cellular targets including Bak, Fas-associated death domain-containing protein (FADD) and procaspase 8, are degraded by E6/E6AP causing inhibition of apoptosis. E6 also inhibits immune response by interacting with host IRF3 and TYK2. These interactions prevent IRF3 transcriptional activities and inhibit TYK2-mediated JAK-STAT activation by interferon alpha resulting in inhibition of the interferon signaling pathway. |
| Application Dilution | |
| WB | =1:500-2000 |
| IHC-P | IHC-P=1:100-500 |
| IHC-F | IHC-F=1:400-800 |
| IF | IF=1:100-500 |
| Flow-Cyt | Flow-Cyt=1ug/Test |
Frequently asked questions
Which applications is HPV16 E6 Polyclonal Antibody (bs-49102R) validated for, and at what dilution?
bs-49102R is validated for WB (WB=1:500-2000); IHC-P (IHC-P=1:100-500); IHC-F (IHC-F=1:400-800); IF (IF=1:100-500); Flow-Cyt (Flow-Cyt=1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HPV16 E6 Polyclonal Antibody (bs-49102R) react with?
Tested reactivity: Human, HPV16.
How should bs-49102R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HPV16 E6 Polyclonal Antibody (bs-49102R)?
Recombinant HPV16 E6