Lane 1: SARS-CoV N protein(Cat# bs-49002P) at 50ng; Lane 2: SARS-CoV N protein(Cat# bs-49002P) at 25ng; Lane 3: SARS-CoV N protein(Cat# bs-49002P) at 15ng; Lane 4: SARS-CoV N protein(Cat# bs-49002P) at 10ng ; Lane 5: SARS-CoV N protein(Cat# bs-49002P) at 5ng;Lane 6: SARS-CoV N protein(Cat# bs-49002P) at 2.5ng probed with SARS-CoV N protein Polyclonal Antibody, Unconjugated (bs-49002R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.