CK12 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4625R-BF647-100uL |
| Product Name | CK12 Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CK12/Cytokeratin 12 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3859 |
| Subcellular location | Cytoplasm, Secreted, Extracellular matrix |
| Synonyms | 65 kDa cytokeratin; CK 12; CK 3; CK12; CK3; Cytokeratin 12; Cytokeratin 3; K12; K3; keratin 12 Meesmann corneal dystrophy; Keratin 12; Keratin 3; Keratin, type I cytoskeletal 12; K1C12_HUMAN; Keratin, type II cytoskeletal 3; KRT12; KRT3. |
| Background | Cytokeratin 12 is a member of the intermediate filament family of proteins and is a heterotetramer of two type I and two type II keratins. Keratin 3 is specifically expressed in the corneal epithelium with family member KRT12. Cytokeratin 12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Defects in KRT3 and KRT12 are a cause of Meesmann corneal dystrophy (MCD), an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Symptoms occur in adulthood and include rupture of the corneal microcysts that may lead to photophobia, contact lens intolerance and intermittent diminution of visual acuity. Defects in KRT12 are a cause of juvenile epithelial corneal dystrophy of Meesmann (MCD) |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CK12 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-4625R-BF647) validated for, and at what dilution?
bs-4625R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CK12 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-4625R-BF647) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Rabbit.
How should bs-4625R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CK12 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-4625R-BF647)?
KLH conjugated synthetic peptide derived from human CK12/Cytokeratin 12
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-4625R-BF647)?
AbBy Fluor-647 on bs-4625R-BF647: excitation / emission 650nm/665nm.