Recombinant SARS-COV-2 S RBD (N501Y) Protein

$SARS-COV-2 S protein RBD (N501Y) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.\r\n$

SARS-COV-2 S protein RBD (N501Y) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.\r\n

The purity of SARS-COV-2 S protein RBD (N501Y) is greater than 95% as determined by SEC-HPLC.

Human ACE2 captured on protein A chip, can bind SARS-CoV S protein RBD (N501Y), His tag with an affinity constant of 1.7nM as determined in SPR assay (Biacore T200).

$Immobilized SARS-CoV-2 S RBD (N501Y) protein, His Tag at 0.5ug\/ml (100ul\/Well). Dose-response curve for ACE2, Fc tag with the EC50 of 12.7ng\/ml determined by ELISA.$

Immobilized SARS-CoV-2 S RBD (N501Y) protein, His Tag at 0.5ug\/ml (100ul\/Well). Dose-response curve for ACE2, Fc tag with the EC50 of 12.7ng\/ml determined by ELISA.

Applications

WB
ELISA
HPLC

Reactivity

SARS-CoV-2

Overview
Catalog #	bs-46011P
Product Name	Recombinant SARS-COV-2 S RBD (N501Y) Protein
Applications	WB, ELISA, HPLC
Reactivity	SARS-CoV-2
Specifications
Conjugation	Unconjugated
Source	SARS-COV-2 S protein RBD (N501Y) is expressed from mammalian with a His tag and Avi at the C-terminal. It contains Arg319 - Phe541 (N501Y).
Storage Buffer	Lyophilized from 0.22um filtered solution in 20mM PB (pH 7.4). 5% trehalose is added as a protectant before lyophilization.
Storage Condition	The product should be stored at -70°C or -20°C.
Target
Gene ID	QHD43416.1
Background	The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.