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NR0B2 Polyclonal Antibody

Paraformaldehyde-fixed, paraffin-embedded mouse spleen tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) incubation at 37\u00b0C for 20 minutes; Antibody incubation with NROB2 Polyclonal Antibody, Unconjugated (bs-4311R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin-embedded mouse spleen tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) incubation at 37\u00b0C for 20 minutes; Antibody incubation with NROB2 Polyclonal Antibody, Unconjugated (bs-4311R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NR0B2 Polyclonal Antibody, Unconjugated (bs-4311R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NR0B2 Polyclonal Antibody, Unconjugated (bs-4311R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NR0B2 Polyclonal Antibody, Unconjugated (bs-4311R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NR0B2 Polyclonal Antibody, Unconjugated (bs-4311R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Rat Kidney tissue lysates; Lane 2: Rat Small intestine tissue lysates probed with NR0B2 Polyclonal Antibody, Unconjugated (bs-4311R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Rat Kidney tissue lysates; Lane 2: Rat Small intestine tissue lysates probed with NR0B2 Polyclonal Antibody, Unconjugated (bs-4311R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)
  • ICC

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Horse
  • Rabbit
Overview
Catalog # bs-4311R
Product Name NR0B2 Polyclonal Antibody
Applications WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Cow, Horse, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NR0B2
Immunogen Range 31-130/257
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 8431
Swiss Prot Q15466
Subcellular location Cytoplasm, Nucleus
Synonyms SHP; SHP1; Nuclear receptor subfamily 0 group B member 2; Orphan nuclear receptor SHP; Small heterodimer partner; NR0B2
Background Acts as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated trancription complex for NEUROD1.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

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