VE Cadherin Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4310R-100uL |
| Product Name | VE Cadherin Polyclonal Antibody |
| Applications |
FCM
IHC-P
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Cow, Pig, Horse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human VE Cadherin |
| Immunogen Range | 251-320/784 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 1003 |
| Swiss Prot | P33151 |
| Subcellular location | Cell membrane |
| Synonyms | 7B4; CD144; Cadherin-5; 7B4 antigen; Vascular endothelial cadherin; VE-cadherin; CDH5 |
| Background | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. |
| Application Dilution | |
| FCM | 1:20-100 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is VE Cadherin Polyclonal Antibody (bs-4310R) validated for, and at what dilution?
bs-4310R is validated for FCM (1:20-100); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does VE Cadherin Polyclonal Antibody (bs-4310R) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Cow, Pig, Horse.
How should bs-4310R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise VE Cadherin Polyclonal Antibody (bs-4310R)?
KLH conjugated synthetic peptide derived from human VE Cadherin Immunogen range: 251-320/784.