SMAD9 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Anti-SMAD9 Polyclonal Antibody, Unconjugated (bs-4253R) at 1:200 followed by conjugation to the secondary antibody

$Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Skin tissue lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human A549 cell lysates; Lane 6: Human HepG2 cell lysates probed with SMAD9 Polyclonal Antibody, Unconjugated (bs-4253R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Skin tissue lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human A549 cell lysates; Lane 6: Human HepG2 cell lysates probed with SMAD9 Polyclonal Antibody, Unconjugated (bs-4253R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IHC-F
IF
Flow-Cyt

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-4253R
Product Name	SMAD9 Polyclonal Antibody
Applications	WB, IHC-P, IHC-F, IF, Flow-Cyt
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human SMAD9
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	4093
Synonyms	MAD homolog 9; MADH6; Mothers against decapentaplegic; Mothers against decapentaplegic homolog 9; SMAD 9; SMAD family member 9; SMAD, mothers against DPP homolog 9 Drosophila; SMAD8A; SMAD8B; Smad9.
Background	SMAD9 is a member of the MAD-related family of molecules. MAD-related proteins are a recently identified family of intracellular proteins that are thought to be essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily.
Application Dilution
WB	1:500-2000
IHC-P
IHC-F
IF
Flow-Cyt