Sphingomyelin Synthase 1 Antibody

Formalin-fixed and paraffin embedded mouse brain labeled with Anti-Sphingomyelin Synthase 1 Polyclonal Antibody, Unconjugated (bs-4216R) followed by conjugation to the secondary antibody and DAB staining

Lane 1: mouse embryo lysates Lane 2: mouse heart lysates probed with Anti Sphingomyelin Synthase 1 Polyclonal Antibody, Unconjugated (bs-4216R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 49kD. Observed band size: 49kD.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Predicted Reactivity

Human
Mouse
Rat
Dog
Cow
Horse
Chicken
Rabbit

Overview
Catalog #	bs-4216R
Product Name	Sphingomyelin Synthase 1 Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Predicted Reactivity	Human, Mouse, Rat, Dog, Cow, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Sphingomyelin Synthase 1
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	259230
Swiss Prot	Q86VZ5
Subcellular location	Cytoplasm, Cell membrane
Synonyms	MOB; MOB1; SMS1; TMEM23; hmob33; Phosphatidylcholine:ceramide cholinephosphotransferase 1; Medulla oblongata-derived protein; Protein Mob; Sphingomyelin synthase 1; Transmembrane protein 23; SGMS1
Background	Sphingomyelin synthases synthesize the sphingolipid, sphingomyelin, through transfer of the phosphatidyl head group, phosphatidylcholine, on to the primary hydroxyl of ceramide. The reaction is bidirectional depending on the respective levels of the sphingolipid and ceramide. Golgi apparatus SMS1 directly and specifically recognizes the choline head group on the substrate, requiring two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. Major form in macrophages. Required for cell growth in certain cell types such as HeLa cells. Suppresses BAX-mediated apoptosis and also prevents cell death in response to stimuli such as hydrogen peroxide, osmotic stress, elevated temperature and exogenously supplied sphingolipids. May protect against cell death by reversing the stress-inducible increase in levels of proapoptotic ceramide.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200