GFAP Polyclonal Antibody

$Lane 1: Human U87MG cell lysates; Lane 2: Human U251 cell lysates probed with GFAP Polyclonal Antibody, Unconjugated (bs-42001R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human U87MG cell lysates; Lane 2: Human U251 cell lysates probed with GFAP Polyclonal Antibody, Unconjugated (bs-42001R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$25 ug total protein per lane of various lysates (see on figure) probed with GFAP polyclonal antibody, unconjugated (bs-42001R) at 1:20000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with GFAP polyclonal antibody, unconjugated (bs-42001R) at 1:20000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$4% Paraformaldehyde-fixed U87-MG (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (GFAP) polyclonal Antibody, unconjugated (bs-42001R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.$

4% Paraformaldehyde-fixed U87-MG (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (GFAP) polyclonal Antibody, unconjugated (bs-42001R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Applications

WB
ELISA
ICC/IF

Reactivity

Human

Predicted Reactivity

Mouse
Rat

Overview
Catalog #	bs-42001r
Product Name	GFAP Polyclonal Antibody
Applications	WB, ELISA, ICC/IF
Reactivity	Human
Predicted Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human GFAP protein
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2670
Swiss Prot	P14136
Subcellular location	Cytoplasm
Synonyms	Astrocyte Marker; FLJ45472; GFAP; Glial Fibrillary Acidic Protein; Intermediate filament protein; GFAP_HUMAN.
Application Dilution
WB	=1:5000-20000
ELISA	ELISA=1:5000-10000
ICC/IF	ICC/IF=1:50-200