EHHADH Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4058R-100uL |
| Product Name | EHHADH Polyclonal Antibody |
| Applications |
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Horse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human EHHADH |
| Immunogen Range | 631-723/723 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 158067 |
| Subcellular location | Cytoplasm |
| Synonyms | 3 hydroxyacyl CoA dehydrogenase; 3,2 trans enoyl CoA isomerase; ECHD antibody Enoyl Coenzyme A, hydratase/3 hydroxyacyl Coenzyme A dehydrogenase; L 3 hydroxyacyl CoA dehydrogenase; L bunctional protein, peroxisomal; L PBE; LBFP; LBP; MGC120586; PBE; PBFE; Peroxisomal bunctional enzyme; Peroxisomal enoyl CoA hydratase. |
| Background | EHHADH is one of three preoxisomal enzymes of the classic peroxisomal Beta-oxidation cycle involved in the catabolism of free fatty acids.Most dietary long chain fatty acids are oxidized in the mitochondria, but very-long-chain fatty acids (VLCFAs) must be shortened first by peroxisomal Beta-oxidation prior to complete oxidation in mitochondria. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is EHHADH Polyclonal Antibody (bs-4058R) validated for, and at what dilution?
bs-4058R is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does EHHADH Polyclonal Antibody (bs-4058R) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Horse.
How should bs-4058R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise EHHADH Polyclonal Antibody (bs-4058R)?
KLH conjugated synthetic peptide derived from human EHHADH Immunogen range: 631-723/723.