ADCY10 Polyclonal Antibody

Formalin-fixed and paraffin embedded human liver cancer labeled with Anti ADCY10 Polyclonal Antibody, Unconjugated (bs-3916R) followed by conjugation to the secondary antibody and DAB staining

$Formalin-fixed and paraffin-embedded rat brain labeled with Anti ADCY10 Polyclonal Antibody, Unconjugated(bs-3916R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.$

Formalin-fixed and paraffin-embedded rat brain labeled with Anti ADCY10 Polyclonal Antibody, Unconjugated(bs-3916R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.

$Lane 1: Human U-87 MG cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human Jurkat cell lysates probed with ADCY10 Polyclonal Antibody, Unconjugated (bs-3916R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Human U-87 MG cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human Jurkat cell lysates probed with ADCY10 Polyclonal Antibody, Unconjugated (bs-3916R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Rat

Predicted Reactivity

Mouse
Dog
Cow
Pig
Horse
Rabbit

Overview
Catalog #	bs-3916R
Product Name	ADCY10 Polyclonal Antibody
Applications	ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human, Rat
Predicted Reactivity	Mouse, Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ADCY10
Immunogen Range	1101-1200/1610
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	55811
Swiss Prot	Q96PN6
Subcellular location	Cytoplasm, Cell membrane
Synonyms	SAC; HCA2; SACI; Sacy; hsAC; Adenylate cyclase type 10; AH-related protein; Adenylate cyclase homolog; Germ cell soluble adenylyl cyclase; Testicular soluble adenylyl cyclase; ADCY10
Background	Soluble adenylyl cyclase that has a critical role in mammalian spermatogenesis. Produces the cAMP which mediates in part the cAMP-responsive nuclear factors indispensable for maturation of sperm in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization. May be the bicarbonate sensor. Involved in ciliary beat regulation.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200