MSH6 Polyclonal Antibody

ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.", "Category"=>"Primary Antibody", "Clonality"=>"Polyclonal", "Concentration"=>"1ug/ul", "Conjugation"=>"Unconjugated", "Cross Reactive Species"=>"Human, Rat", "Datasheet URL"=>"https://www.biossusa.com/store/datasheets/bs-3804R", "Gene ID"=>"2956", "Gene ID Link"=>"Entrez Gene: 2956", "Host"=>"Rabbit", "images"=>"[{\"id\":\"9133\",\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":\"1\",\"origin_notes\":\"\",\"product_id\":\"6823\",\"productconjugation_id\":null,\"application_id\":\"4\",\"application_note\":\"\",\"dilution\":\"\",\"cross_reactive_species_id\":\"1\",\"cross_reactive_species_note\":\"\",\"description\":\"Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-MSH6 Polyclonal Antibody, Unconjugated(bs-3804R) 1:200 followed by conjugation to the secondary antibody and DAB staining\\\\n\",\"sample_type_id\":\"2\",\"sample_details\":null,\"rating\":\"3\",\"disease_id\":\"1\",\"sample_tissue_id\":\"12\",\"cell_line_id\":null,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"6823\\/9133_raw.jpg\",\"filepath_med\":\"6823\\/9133_med.jpg\",\"filepath_thumb\":\"6823\\/9133_thumb.jpg\",\"display_order\":\"0\",\"lock_version\":\"1\",\"created_at\":\"2016-06-06 22:15:36\",\"updated_at\":\"2016-06-06 22:15:36\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/9133\"},{\"id\":\"16510\",\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":\"1\",\"origin_notes\":\"\",\"product_id\":\"6823\",\"productconjugation_id\":null,\"application_id\":\"4\",\"application_note\":\"\",\"dilution\":\"1:400\",\"cross_reactive_species_id\":\"1\",\"cross_reactive_species_note\":\"\",\"description\":\"Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\\u00b0C for 20min; Antibody incubation with MSH6 Polyclonal Antibody (bs-3804R) at 1:400 overnight at 4\\u00b0C, followed by a conjugated secondary and DAB staining.\",\"sample_type_id\":\"2\",\"sample_details\":null,\"rating\":\"2\",\"disease_id\":null,\"sample_tissue_id\":\"6\",\"cell_line_id\":null,\"protocols_used\":\"Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\\u00b0C for 30min; Antibody incubation with (MSH6) Polyclonal Antibody, Unconjugated (bs-3804R) at 1:400 overnight at 4\\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.\",\"additional_notes\":\"\",\"filepath_raw\":\"6823\\/16510_raw.jpg\",\"filepath_med\":\"6823\\/16510_med.jpg\",\"filepath_thumb\":\"6823\\/16510_thumb.jpg\",\"display_order\":\"3\",\"lock_version\":\"1\",\"created_at\":\"2017-06-12 22:00:03\",\"updated_at\":\"2017-06-12 22:00:03\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/16510\"}]", "Immunogen Sequence"=>"812-910/1360", "Isotype"=>"IgG", "Lead Time"=>"3 to 5 business days.", "Modification"=>"Unmodified", "Predicted Reactivity"=>"Mouse, Dog, Cow, Sheep, Pig, Rabbit", "Product URL"=>"https://www.biossusa.com/products/bs-3804R", "Purification"=>"Purified by Protein A.", "Size"=>"100ul", "Source"=>"KLH conjugated synthetic peptide derived from human MSH6", "Storage"=>"0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.", "Storage Buffer"=>"0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.", "Storage Condition"=>"Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.", "Subcellular Locations"=>"Nucleus", "Swiss Prot"=>"P52701", "Swiss Prot Link"=>"Swiss Prot: P52701", "Synonyms"=>"GTBP; HSAP; p160; GTMBP; HNPCC5; DNA mismatch repair protein Msh6; hMSH6; G/T mismatch-binding protein; MutS-alpha 160 kDa subunit; MSH6", "Target Protein"=>"MSH6"} -->

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
  • Dog
  • Cow
  • Sheep
  • Pig
  • Rabbit
Overview
Catalog # bs-3804R
Product Name MSH6 Polyclonal Antibody
Applications WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Human, Rat
Predicted Reactivity Mouse, Dog, Cow, Sheep, Pig, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human MSH6
Immunogen Range 812-910/1360
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 2956
Swiss Prot P52701
Subcellular location Nucleus
Synonyms GTBP; HSAP; p160; GTMBP; HNPCC5; DNA mismatch repair protein Msh6; hMSH6; G/T mismatch-binding protein; MutS-alpha 160 kDa subunit; MSH6
Background Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200