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Claudin 6 Polyclonal Antibody, FITC Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Pig
  • Horse
  • Rabbit
  • GuineaPig
Overview
Catalog # bs-3754R-FITC-100uL
Product Name Claudin 6 Polyclonal Antibody, FITC Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human, Pig, Horse, Rabbit, GuineaPig
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Claudin 6
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 9074
Subcellular location Cell membrane
Synonyms Claudin6; CLDN 6; CLDN6; Skullin 2; CLD6_HUMAN.
Background Claudin 6 is a multipass transmembrane protein in the Claudin family. Claudin 6 is expressed by epithelial cells where it participates in tissue development and the maintenance of tight junction integrity. Claudin 6 is also a global marker of definitive endoderm. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Claudin 6 Polyclonal Antibody, FITC Conjugated (bs-3754R-FITC) validated for, and at what dilution?

bs-3754R-FITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Claudin 6 Polyclonal Antibody, FITC Conjugated (bs-3754R-FITC) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Pig, Horse, Rabbit, GuineaPig.

How should bs-3754R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Claudin 6 Polyclonal Antibody, FITC Conjugated (bs-3754R-FITC)?

KLH conjugated synthetic peptide derived from human Claudin 6

What are the excitation and emission wavelengths of the FITC conjugate (bs-3754R-FITC)?

FITC on bs-3754R-FITC: excitation / emission 494nm/518nm.

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