TCTP Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TCTP Polyclonal Antibody, Unconjugated (bs-3578R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TCTP Polyclonal Antibody, Unconjugated (bs-3578R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Sheep
Pig
Horse
Rabbit
Guinea Pig

Overview
Catalog #	bs-3578R
Product Name	TCTP Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit, Guinea Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human TCTP
Immunogen Range	85-172/172
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7178
Subcellular location	Cytoplasm
Synonyms	FLJ27337; Fortilin; Histamine releasing factor; Histamine-releasing factor; HRF; Immunoglobulin E-dependent; p02; p23; TCTP; TCTP_HUMAN; TPT1; Translationally controlled tumor protein; Translationally-controlled tumor protein; Tumor protein translationally controlled 1; tumor protein translationally-controlled 1; tumor protein, translationally-controlled 1.
Background	TCTP is involved in calcium binding and microtubule stabilization. It is an immunoglobulin E dependent histamine-releasing factor produced by lymphocytes of atopic children and present in biologic fluids of allergic patients. Both human and mouse recombinant HRF proteins caused histamine release from human basophils of a subpopulation of donors (referenced from OMIM).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200

Applications

Reactivity

Predicted Reactivity

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