Nur77 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Anti Nur77 Polyclonal Antibody (bs-3513R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

$Sample: \r\nLane 1: Human Jurkat cell lysates \r\nLane 2: Human HeLa cell lysates \r\nPrimary: Anti-Nur77 (bs-3513R) at 1\/1000 dilution \r\nSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution \r\nPredicted band size: 66 kDa \r\nObserved band size: 73 kDa \r\nLot: AI11268723\r\n$

Sample: \r\nLane 1: Human Jurkat cell lysates \r\nLane 2: Human HeLa cell lysates \r\nPrimary: Anti-Nur77 (bs-3513R) at 1\/1000 dilution \r\nSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution \r\nPredicted band size: 66 kDa \r\nObserved band size: 73 kDa \r\nLot: AI11268723\r\n

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse
Rat

Predicted Reactivity

Human

Overview
Catalog #	bs-3513R
Product Name	Nur77 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Mouse, Rat
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Nur77/GFRP
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3164
Swiss Prot	P22736
Subcellular location	Nucleus
Synonyms	HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77; Nuclear receptor subfamily 4 group A member 1; Early response protein NAK1; Nuclear hormone receptor NUR/77; Orphan nuclear receptor HMR; Orphan nuclear receptor TR3; ST-59; Testicular receptor 3; NR4A1; NAK1
Background	Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200