FCGR1A/CD64 Polyclonal Antibody

Name: FCGR1A/CD64 Polyclonal Antibody
Brand: Bioss
SKU: bs-3511R
Price: 375.0 USD
Availability: InStock

$Human 293T cells probed with CD64\/IGFR1 Polyclonal Antibody, Unconjugated (bs-3511R-A647) at 5ug for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).$

Human 293T cells probed with CD64\/IGFR1 Polyclonal Antibody, Unconjugated (bs-3511R-A647) at 5ug for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

$293T cells probed with CD64\/IGFR1 Polyclonal Antibody, ALEXA FLUOR\u00ae 647 Conjugated (bs-3511R-A647) at 1:20 for 30 minutes compared to control cells (blue) and isotype control (orange).$

293T cells probed with CD64\/IGFR1 Polyclonal Antibody, ALEXA FLUOR\u00ae 647 Conjugated (bs-3511R-A647) at 1:20 for 30 minutes compared to control cells (blue) and isotype control (orange).

$Raw264.7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD64 Antibody(bs-3511R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).\r\n$

Raw264.7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD64 Antibody(bs-3511R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).\r\n

$25 ug total protein per lane of various lysates (see on figure) probed with FCGR1A\/CD64 polyclonal antibody, unconjugated (bs-3511R) at 1:300 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with FCGR1A\/CD64 polyclonal antibody, unconjugated (bs-3511R) at 1:300 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
Flow-Cyt

Reactivity

Mouse

Overview
Catalog #	bs-3511R-100uL
Product Name	FCGR1A/CD64 Polyclonal Antibody
Applications	WB FLOW-CYT
Reactivity	Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CD64/IGFR1
Immunogen Range	101-200/374
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2209
Swiss Prot	P12314
Subcellular location	Cell membrane
Synonyms	CD64; CD64A; FCG1; FCGR1; FCRI; FcgammaRI; IGFR1; FCGR1_HUMAN; FCGR1A; IgG Fc receptor I; Fc-gamma RI (FcRI); Fc-gamma RIA (FcgammaRIa); Fc gamma receptor Ia; Fc fragment of IgG, high affinity Ia, receptor for (CD64); Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc fragment of IgG receptor Ia; Fc gamma RI
Background	High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses.
Application Dilution
WB	1:500-2000
Flow-Cyt	1ug/Test

Frequently asked questions

Which applications is FCGR1A/CD64 Polyclonal Antibody (bs-3511R) validated for, and at what dilution?

bs-3511R is validated for WB (1:500-2000); Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does FCGR1A/CD64 Polyclonal Antibody (bs-3511R) react with?

Tested reactivity: Mouse.

How should bs-3511R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise FCGR1A/CD64 Polyclonal Antibody (bs-3511R)?

KLH conjugated synthetic peptide derived from human CD64/IGFR1 Immunogen range: 101-200/374.

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FCGR1A/CD64 Polyclonal Antibody

Applications

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Reactivity

BiossPM Rainbow Protein Marker (10~180 kDa)

BiossPM Rainbow Protein Marker (10~245 kDa)

BiossPM Rainbow Protein Marker (10~310 kDa)

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