CD105 Polyclonal Antibody

Name: CD105 Polyclonal Antibody
Brand: Bioss
SKU: bs-34063R
Price: 375.0 USD
Availability: InStock

$Lane 1: Human Huvec cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human U87MG cell lysates probed with CD105 Polyclonal Antibody, Unconjugated (bs-34063R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Human Huvec cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human U87MG cell lysates probed with CD105 Polyclonal Antibody, Unconjugated (bs-34063R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

$Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD105 Polyclonal Antibody, Unconjugated (bs-34063R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD105 Polyclonal Antibody, Unconjugated (bs-34063R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Serous ovarian cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD105 Polyclonal Antibody, Unconjugated (bs-34063R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Serous ovarian cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD105 Polyclonal Antibody, Unconjugated (bs-34063R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD105 Polyclonal Antibody, Unconjugated (bs-34063R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD105 Polyclonal Antibody, Unconjugated (bs-34063R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD105 Polyclonal Antibody, Unconjugated (bs-34063R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD105 Polyclonal Antibody, Unconjugated (bs-34063R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD105 Polyclonal Antibody, Unconjugated (bs-34063R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD105 Polyclonal Antibody, Unconjugated (bs-34063R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD105 Polyclonal Antibody, Unconjugated (bs-34063R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD105 Polyclonal Antibody, Unconjugated (bs-34063R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Applications

WB
IHC-F
IF
IHC-P

Reactivity

Human

Overview
Catalog #	bs-34063r-100uL
Product Name	CD105 Polyclonal Antibody
Applications	WB IHC-F IF IHC-P
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CD105
Immunogen Range	351-450/625
Clonality	Polyclonal
Isotype	IgG
Concentration	Lot Dependent
Purification	purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2022
Swiss Prot	P17813
Synonyms	END; Endoglin; ENG; FLJ41744; HHT1; ORW; ORW1; Osler Rendu Weber syndrome 1; RP11 228B15.2; CD 105; CD105 antigen; EGLN_HUMAN; AI528660; AI662476; S-endoglin; SN6.
Background	Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
Application Dilution
WB	1:500-2000
IHC-F	1:100-500
IF	1:100-500
IHC-P	1:100-500

Frequently asked questions

Which applications is CD105 Polyclonal Antibody (bs-34063R) validated for, and at what dilution?

bs-34063R is validated for WB (1:500-2000); IHC-F (1:100-500); IF (1:100-500); IHC-P (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CD105 Polyclonal Antibody (bs-34063R) react with?

Tested reactivity: Human.

How should bs-34063R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CD105 Polyclonal Antibody (bs-34063R)?

KLH conjugated synthetic peptide derived from human CD105 Immunogen range: 351-450/625.

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CD105 Polyclonal Antibody

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Reactivity

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