Mst1(Thr183)/Mst2(Thr180) Antibody

$Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti Phospho-Mst1(Thr183)\/Mst2(Thr180) Polyclonal Antibody, Unconjugated (bs-3294R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti Phospho-Mst1(Thr183)\/Mst2(Thr180) Polyclonal Antibody, Unconjugated (bs-3294R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Hela cell lysates probed with Phospho-Mst1(Thr183) + Mst2(Thr180) Polyclonal Antibody, Unconjugated (bs-3294R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

HepG2 cell lysates probed with Phospho-Mst1(Thr183) + Mst2(Thr180) Polyclonal Antibody, Unconjugated (bs-3294R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Mouse spleen were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Mst1(Thr183)\/Mst2(Thr180) Antibody(bs-3294R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).$

Mouse spleen were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Mst1(Thr183)\/Mst2(Thr180) Antibody(bs-3294R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Horse
Chicken
Rabbit

Overview
Catalog #	bs-3294R
Product Name	Mst1(Thr183)/Mst2(Thr180) Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human Mst1 around the phosphorylation site of Thr183
Modification Site	Thr 183/Thr180
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6789, 6788
Swiss Prot	Q13043, Q13188
Subcellular location	Nucleus
Synonyms	MST1; Serine/threonine-protein kinase 4, Mammalian STE20-like protein kinase 1, MST-1, STE20-like kinase MST1, Serine/threonine-protein kinase Krs-2 MST2: Serine/threonine-protein kinase 3, Mammalian STE20-like protein kinase 2, MST-2, STE20-like kinase MST2, Serine/threonine-protein kinase Krs-1
Background	Protein which catalyzes the phosphorylation of serine or threonine residues on target proteins by using ATP as phosphate donor. Such phosphorylation may cause changes in the function of the target protein. Protein kinases share a conserved catalytic core common to both serine/ threonine and tyrosine protein kinases.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200

Applications

Reactivity

Predicted Reactivity

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