MYPT1 (Thr696) Polyclonal Antibody

Applications

  • WB
  • FCM

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
  • Dog
  • Cow
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-3287R
Product Name MYPT1 (Thr696) Polyclonal Antibody
Applications WB, FCM
Specificity This phosphorylation site is homologous to that of Thr694 in Mouse and Thr697 in Rat. The region containing this phosphorylation site is known to be homologous to that of the related protein, PP12C, and will react with this protein when phosphorylated at Thr560 in Human and Mouse, and Thr561 in Rat.
Reactivity Human, Rat
Predicted Reactivity Mouse, Dog, Cow, Horse, Chicken, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human around the phosphorylation site of Ser696
Modification Site Thr696
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 4659
Swiss Prot O14974
Subcellular location Cytoplasm
Synonyms MBS; M130; MYPT1; Protein phosphatase 1 regulatory subunit 12A; Myosin phosphatase-targeting subunit 1; Myosin phosphatase target subunit 1; Protein phosphatase myosin-binding subunit; PPP1R12A
Background Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.
Application Dilution
WB 1:300-5000
FCM 1:20-100