Phospho-IKB beta (Ser23) Polyclonal Antibody
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-3191R-100uL |
| Product Name | Phospho-IKB beta (Ser23) Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human NFKBIB around the phosphorylation site of Ser23 |
| Modification Site | Ser23 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 4793 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | IKB beta Ser23; IKB beta S23; p-IKB beta Ser23; p-IKB beta S23; NFKBIBSer23; NFKBIBphospho S23; IkB-betaSer22; IkB beta; NF-kappa-B inhibitor beta; IKB beta; NF-kappa-BIB; TRIP-9; I-kappa-B-beta; IkappaBbeta; IkB-beta; IkB-B; Thyroid receptor-interacting protein 9; TR-interacting protein 9; NFKBIB; IKBB. |
| Background | The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jul 2011]. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-IKB beta (Ser23) Polyclonal Antibody (bs-3191R) validated for, and at what dilution?
bs-3191R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-3191R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-IKB beta (Ser23) Polyclonal Antibody (bs-3191R)?
KLH conjugated synthetic phosphopeptide derived from human NFKBIB around the phosphorylation site of Ser23