FoxO3a (Ser318 + Ser321) Antibody

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
Overview
Catalog # bs-3138R
Product Name FoxO3a (Ser318 + Ser321) Antibody
Applications WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity These phosphorylation sites are homologous to Ser317 + Ser320 in Mouse and Rat. Due to the highly conserved nature of this sequence, there is a chance that this antibody will react with FoxO1a when phosphorylated at the the sites of Ser322 and Ser325 in Human, Ser319 and Ser322 in Mouse, and Ser316 and Ser319 in Rat.
Reactivity Human, Rat
Predicted Reactivity Mouse, Dog, Cow, Sheep, Pig, Horse, Chicken
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human FoxO3a around the phosphorylation site of Ser318/321
Modification Site Ser318 + Ser321
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 2309
Swiss Prot O43524
Subcellular location Cytoplasm, Nucleus
Synonyms FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2; Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1; FOXO3
Background Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34C expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200